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After incubating the mixture at 25 C for 10 min, 100 l of 0.5% N-ethylmaleimide (NEM), 200 l of 0.61 M TCA, 200 l of 0.8 M orthophosphoric acid, and 200 l of 5.25% 2,2-bipyridyl (dissolved in 70% ethanol), 100 l of FeCl 3 were added

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